Introduction
MitoTrack™ Green FM (Known as MitoTracker® Green FM, Trade Mark of ThermoFisher Scientific) is an green-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is not well-retained after aldehyde fixation.
| Specifications: |
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| Excitation/Emission: | 490/516 nm | |
| Shipping Condition: | Ambient | |
| Storage Conditions: | -20ºC, protect from light | |
| Molecular Formula: | C34H28Cl5N3O | |
| Molecular Weight: | 671.88 | |
| CAS Number: | N/A | |
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To Order
Documents
| Protocol (PDF): | C040 |
| MSDS (PDF): | MSDS-C040 |
| COA (PDF): | C040 |
Reference
Shim SH, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, Wang X, Xu C, Bi GQ, Zhuang X,
Proc Natl Acad Sci U S A (2012) 109:13978-13983
Proc Natl Acad Sci U S A (2012) 109:13978-13983
Lipscomb EA, Sarmiere PD, Freeman RS
J Biol Chem (2001) 276:5085-5092
J Biol Chem (2001) 276:5085-5092
Frequently asked questions (FAQs)
It looks like my Mitotracker™ dye is staining more than just the mitochondria. Why?
This is typically a result of using too high of a concentration of the MitoViewr™ dye. Most organic dyes are used in the low micromolar range. The MitoView™ dyes are used at a much lower concentration, around 50–200 nanomolar. Higher concentrations can cause background fluorescence and non-mitochondrial staining.