Introduction
BCECF, AM is an ideal cell-permeant, dual-excitation ratiometric pH indicator for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm. This dye is provided as 1 mg solid (C028) and 1 mg/mL solution in DMSO (C029).
| Specifications: |
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| Excitation/Emission: | 490/535 (after cleavage) | |
| Shipping Condition: | Ice pack | |
| Storage Conditions: | -20ºC, protect from light | |
| Molecular Formula: | N/A | |
| Molecular Weight: | N/A | |
| CAS Number: | 117464-70-7 | |
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To Order
Documents
| Protocol (PDF): | C028 | C029 |
| MSDS (PDF): | MSDS-C028 | MSDS-C029 |
| COA (PDF): | C028 | C029 |
Reference:
Jayadev S, Petranka JG, Cheran SK, Biermann JA, Barrett JC, Murphy E
J Biol Chem (1999) 274:8261-8268
J Biol Chem (1999) 274:8261-8268
Parish CR
Immunol Cell Biol (1999) 77:499-508
Immunol Cell Biol (1999) 77:499-508
Iizuka T, Tanaka T, Suematsu M, Miura S, Watanabe T, Koike R, Ishimura Y, Ishii H, Miyasaka N, Miyasaka M
J Immunol (2000) 164:2463-2471
J Immunol (2000) 164:2463-2471