CDCFDA, SE is a fixable-cell-permeant, fluorescein-based tracer for very long-term cell labeling. CDCFDA, SE passively diffuse into cells and it is nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the highly fluorescent, amine-reactive fluorophore. Upon reaction with amine-containing residues of intracellular proteins, this probe is well retained in cells.
| Specifications: |
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| Excitation/Emission: | 504/529 nm (after cleavage) | |
| Shipping Condition: | Ice pack | |
| Storage Conditions: | -20ºC, protect from light | |
| Molecular Formula: | C29H17Cl2NO11 | |
| Molecular Weight: | 626.35 | |
| CAS Number: | 147265-60-9 | |
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| Protocol (PDF): | C036 |
| MSDS (PDF): | MSDS-C036 |
| COA (PDF): | C036 |
Reference:
Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae.
Shiflett SL, Ward DM, Huynh D, Vaughn MB, Simmons JC, Kaplan J
J Biol Chem (2004) 279:10982-10990
Degradation of HMG-CoA reductase-induced membranes in the fission yeast, Schizosaccharomyces pombe.
Lum PY, Wright R
J Cell Biol (1995) 131:81-94