Introduction
5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-carboxyfluorescein diacetate yields 5(6)-carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells.
| Specifications: |
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| Excitation/Emission: | 490/520 (after cleavage) | |
| Shipping Condition: | Ambient | |
| Storage Conditions: | -20ºC, protect from light | |
| Molecular Formula: | C25H16O9 | |
| Molecular Weight: | 460.40 | |
| CAS Number: | 124387-19-5 | |
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To Order
Documents
| Protocol (PDF): | C031 |
| MSDS (PDF): | MSDS-C031 |
| COA (PDF): | C031 |
References:
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Breeuwer P, et al.
Appl Environ Microbiol (1997) 63:178-178
Breeuwer P, et al.
Appl Environ Microbiol (1997) 63:178-178
Fluorescent microscopic system for detection of microorganisms and organaic compounds on Mars.
Saito T, Kawasaki Y, Kaneko T, Kobayashi K, Miyakawa A
Proc SPIE-Int Soc Opt Eng (1999) 3755:24-32
Proc SPIE-Int Soc Opt Eng (1999) 3755:24-32
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Parish CR
Immunol Cell Biol (1999) 77:499-508
Immunol Cell Biol (1999) 77:499-508