Monobromobimane (mBBr) | ABP Biosciences

Introduction

Monobromobimane (mBBr) is essentially nonfluorescent, and freely passively diffuse across the plasma membrane into the cytoplasm, where they form blue-fluorescent adducts with intracellular glutathione and thiol containing proteins. The glutathione conjugate of monobromobimane has absorption/emission maxima ~394/490 nm. mBBr has been reported to be used as effective tracer of gap-junctional communication.

Specifications:
Excitation/Emission:	385/463 nm
Shipping Condition:	Ambient
Storage Conditions:	-20ºC, protect from light
Molecular Formula:	C10H11BrN2O2
Molecular Weight:	271.11
CAS Number:	71418-44-5

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Documents

Protocol (PDF):	C038
MSDS (PDF):	MSDS-C038
COA (PDF):	COA-C038

Reference:

Formation of S-nitrosothiols via direct nucleophilic nitrosation of thiols by peroxynitrite with elimination of hydrogen peroxide.

van der Vliet A, Hoen PA, Wong PS, Bast A, Cross CE
J Biol Chem (1998) 273:30255-30262

Orientation of actin monomer in the F-actin filament: radial coordinate of glutamine-41 and effect of myosin subfragment 1 binding on the monomer orientation.

Kasprzak AA, Takashi R, Morales MF
Biochemistry (1988) 27:4512-4522

Structure of actin observed by fluorescence resonance energy transfer spectroscopy.

Miki M, O'Donoghue SI, Dos Remedios CG
J Muscle Res Cell Motil (1992) 13:132-145

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C038	Monobromobimane (mBBr)	385/463	25 mg	800