Introduction
5(6)-CDCFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-CDCFDA yields the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pKa of 4.8, this dye can be used as an acidic pH sensor.
| Specifications: |
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| Excitation/Emission: | 504/529 nm (after cleavage) | |
| Shipping Condition: | Ambient | |
| Storage Conditions: | -20ºC, protect from light | |
| Molecular Formula: | C25H14Cl2O9 | |
| Molecular Weight: | 529.29 | |
| CAS Number: | 127770-45-0 | |
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