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SYTO 13 Green Nucleic Acid Stain

Introduction

SYTO 13 Green Nucleic Acid Stain is a cell-permeant nucleic acid stain that shows a large fluorescence enhancement upon binding nucleic acids. The SYTO 13 dye can be used to stain RNA and DNA in both live and dead eukaryotic cells, as well as in Gram-positive and Gram-negative bacteria. Eukaryotic cells incubated with SYTO 13 dye generally show cytoplasmic or mitochondrial staining, as well as nuclear staining. This dye is provided as 5 mM solution in DMSO.


Features:
 

  • Permeability to virtually all cell membranes, including mammalian cells and bacteria.
  • High molar absorptivity, with extinction coefficients >50,000 cm-1 M-1 at visible absorption maxima.
  • Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when not bound to nucleic acids.
  • Quantum yields that are typically >0.4 when bound to nucleic acids.

 

Specifications:  
Excitation/Emission: RNA 491/514 nm; DNA 488/509 nm
Shipping Condition: Ambient
Storage Conditions: -20ºC, protect from light
Molecular Formula: C25H21ClN2O5S
Molecular Weight: 496.96
Concentration: 5 mM in DMSO

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C082 SYTO 13 Green Nucleic Acid Stain   250 µL 1500 

Documents

 

Protocol (PDF): C082
MSDS (PDF):  MSDS-C082
COA (PDF):  C082